Results
| PMID | 22633261 |
| Gene Name | PTX3 |
| Condition | Endometriosis (ovarian) |
| Association |
Associated |
| Sex | Female |
| Other associated phenotypes |
Ovarian endometriosis |
|
Fertil Steril. 2012 Aug;98(2):415-22.e1-12. doi: Kobayashi, Hiroharu| Yamashita, Yoriko| Iwase, Akira| Yoshikawa, Yutaka| Yasui, Hiroyuki| Kawai, Yoshichika| Uchida, Koji| Uno, Nozomi| Akatsuka, Shinya| Takahashi, Takashi| Kikkawa, Fumitaka| Toyokuni, Shinya Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Showa-ku, Nagoya, Japan. OBJECTIVE: To understand the role of ectopic endometriotic stromal cells in ovarian endometriosis (OEM) and the associated risks for infertility and carcinogenesis. DESIGN: Analyses of secreted proteins and gene expression using immortalized eutopic/ectopic endometrial(-otic) stromal cells from OEM. SETTING: University. PATIENT(S): Women with and without OEM. INTERVENTION(S): Samples of endometrial(-otic) tissue from women with or without OEM. MAIN OUTCOME MEASURE(S): Immunohistochemical analysis of oxidative stress in OEM, gene expression profiles, and the identification of secreted proteins by mass spectrometry in immortalized endometrial(-otic) stromal cells. RESULT(S): 4-Hydroxy-2-nonenal-modified proteins and carboxymethyllysine were abundant in the stroma, rather than epithelia, of OEM patients, indicating the presence of oxidative stress. Immortalized ectopic endometriotic stromal cells exhibited high IRP1/IRP2/HIF-1beta expression and contained lower amounts of iron and copper than their eutopic counterparts. Expression profiles, in combination with protein identification, revealed that complement component 3 (C3) and pentraxin-3 (PTX3) are the major proteins secreted from immortalized ectopic endometriotic stromal cells. Complement-3/PTX3 promoted the secretion of various cytokines by THP1 macrophage cells and thus supported M1 differentiation. CONCLUSION(S): Immortalized ectopic endometriotic stromal cells in OEM predominantly secrete C3 and PTX3 and exhibit a differential regulation of iron metabolism. Mesh Terms: C-Reactive Protein/physiology| Cell Line, Transformed| Cells, Cultured| Choristoma/*metabolism/pathology| Complement C3/physiology| Endometriosis/*metabolism/pathology| *Endometrium/cytology/immunology/metabolism| Female| Humans| Immunomodulation |
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